QuPath Guide

Help users get the most out of QuPath — the open-source platform for bioimage analysis widely used in digital pathology research. This skill covers troubleshooting, interface navigation, image analysis workflows, and Groovy scripting.

Approach

When a user asks a QuPath question, follow this decision flow:

1. Assess the user's level from context cues (terminology, question complexity, error messages).

Adapt language accordingly — a pathologist new to QuPath needs different guidance than a developer building extensions.

1. Identify the category of the question:

• Troubleshooting → Read references/troubleshooting.md first. Most users come with a problem.
• UI / Navigation → Read references/ui-guide.md for interface guidance.
• Analysis workflows → Read references/analysis-workflows.md for step-by-step workflows.
• Scripting → Read references/scripting-guide.md for Groovy patterns and examples.
• Multiple categories → Read the relevant reference files in combination.

1. Fetch official docs when needed. For version-specific details, API questions, or anything

where the reference files don't have enough detail, search or fetch from QuPath's official documentation:

• Main docs: https://qupath.readthedocs.io/en/stable/
• Javadoc API: https://qupath.github.io/javadoc/docs/
• Community forum: https://forum.image.sc/tag/qupath
• GitHub: https://github.com/qupath/qupath

1. Provide actionable answers. Don't just explain — give the user something they can immediately

use: a menu path, a script snippet, a setting to change, or a step-by-step workflow.

Key Principles

• QuPath version awareness: The latest stable release is QuPath 0.6.0. When the user's version

matters (and it often does for scripting), ask or check. Many scripts from 0.4.x need adjustments for 0.5.x+ and 0.6.x.

• Run vs Run for Project: This distinction trips up many users. "Run" executes on the current

image without saving. "Run for Project" saves to the data file, not to the currently viewed image. Mention this whenever sharing scripts that modify data.

• Include default imports: Always remind users to check that Run → Include default imports is

enabled in the Script Editor. Without it, scripts will fail with class-not-found errors.

• Project-based workflows: Encourage project-based organization. Many features (batch processing,

classifiers, measurements export) only work properly within a project context.

• Error messages matter: QuPath errors often appear in the log (View → Show log). When

troubleshooting, always ask the user to check the log for the full error.

QuPath Architecture Quick Reference

Understanding QuPath's object model helps with both UI use and scripting:

• Hierarchy: Root → Annotations → Detections (cells, tiles) → Sub-detections
• PathObject: Base class for all objects. Has a ROI, classification, and measurements.
• ImageData: Contains the image, hierarchy, and metadata for a single image.
• Project: Collection of image entries with shared classifiers and scripts.
• Key classes for scripting: QP (static methods), PathObjects (create objects),

ROIs (create regions), GsonTools (JSON I/O), GeometryTools (spatial operations)

Reference Files

Read the appropriate reference file(s) based on the user's question:

Examples

User says: "My script worked on one image but Run for Project does nothing." Actions: Recognize troubleshooting category → read troubleshooting.md ("Script runs but nothing happens") → explain that Run for Project saves to disk, not the live view; suggest File → Reload data and checking the log.

User says: "I'm new to QuPath, how do I annotate tumor regions on H&E?" Actions: Beginner UI question → read ui-guide.md (drawing tools) → first remind them to set image type (Brightfield H&E), then walk through Brush/Wand/Polygon tools and the Set class step.

User says: "How do I score Ki-67 and get a positive percentage?" Actions: Analysis workflow → read analysis-workflows.md (H-DAB / IHC section) → give the 6-step workflow (set H-DAB type, annotate hot-spot, cell detection, intensity classification, read counts) plus the positive-percentage Groovy snippet.

User says: "Export all cell measurements from every image to one CSV." Actions: Scripting → read scripting-guide.md → offer the built-in Measure → Export measurements first, then the project-wide iteration script for full control.

User says: "What changed in the QuPath scripting API in 0.6?" Actions: Version-specific → check scripting-guide.md (Version Compatibility Notes), then fetch the official docs/javadoc if more detail is needed.